DNA

Part:BBa_K2100047:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-19)


pEXPR pERE5:TP901


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 711
    Illegal EcoRI site found at 1814
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 711
    Illegal EcoRI site found at 1814
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 711
    Illegal EcoRI site found at 1814
    Illegal BglII site found at 1594
    Illegal BamHI site found at 298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 711
    Illegal EcoRI site found at 1814
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 711
    Illegal EcoRI site found at 1814
    Illegal NgoMIV site found at 462
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a synthetic promoter with a gene from a mammalian genome.

References